Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-2ug/mlWestern Blot: 0.5-2ug/mlImmunocytochemistry: 5-20ug/mlImmunohistochemistry (frozen sections): 5-20ug/mlImmunohistochemistry (paraffin sections): 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control Included:141069A: ILR6, Positive ControlSuppled as a recombinant protein (Ala19-Val108 ) in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity:Recognizes rat Interleukin 6 Receptor (IL6R)
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to Ala19-Val108 from rat Interleukin 6 Receptor, expressed in E. coli.