Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:1:200Western Blot: 1:50-1:1:400Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher.
Positive Control Included:141049A: IL;35, Positive ControlSuppled as a recombinant IL35 protein in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human Interleukin 35 (IL35)
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:IL35 complex composed of IL27B subunit (Arg21-Lys229) and the mature form of human IL12A (Arg23-Ser219) linked by a polypeptide linker (GGGGS)3 expressed in E. coli.