84-1434-25　Interleukin 1 Beta (IL1b, IL1-B, IL1-Beta, IL1F2, IL-1b, Interleukin-1 Family Member 2) 200ul　140945

特徴

• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100-1:200Western Blot: 1:100-1:400Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (Paraffin): 1:50-1:200 Immunocytochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Positive Control (supplied):140945A: Suppled as a recombinant protein (aa118-268) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Amino Acid Sequence:The target protein is fused with two N-terminal Tags, His-tag and T7-tag expressed in E.
• coli
• MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRS LHCRLQDAQQ KSLVLSGTYE LKALHLNAEN LNQQVVFSMS FVQGEESNDK IPVALGLRGK NLYLSCVMKD DKPTLQLESV DPNRYPKKKM EKRFVFNKIE IKDKLEFESA QFPNWYISTS QTEYMPVFLG NNSGGQDLID FSMEFVSS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.