Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-200Western Blot: 1:100-400Immunocytochemistry: 1:100-500Immunohistochemistry: 1:100-500 (frozen)Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
Quality Control (Supplied):Recombinant human FLG2 (amino acids 1915-2143 ) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
Usage:10ul/well using DAB as substrate5ul/well when used in ECLStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Filaggrin 2 (FLG2).
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to amino acids 1915-2143 of human FLG2, UniProt Accession #Q5D862
