Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:200Western Blot: 1:50-1:400Immunocytochemistry: 1:50-1:500Immunohistochemistry (frozen): 1:50-1:500Immunohistochemistry (paraffin sections): 1:10-1:100Optimal dilutions to be determined by the researcher.
Positive Control (Supplied):139533A: Suppled as a recombinant protein (aa35-361) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)