84-1420-80 Calreticulin (CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60, HACBP, grp60, CRTC, CALR) 200ul 139529
特徴
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:5000Western Blot: 1:50-1:400Immunohistochemistry (frozen): 1:50-1:500Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500Optimal dilutions to be determined by the researcher.
Amino Acid Sequence:MGHHHHHHSGSEF-EPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE EKDKGLQTSQ DARFYALSAS FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPNSLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VES
Positive Control (Supplied):139529A: Suppled as a recombinant protein (Glu18-Ser193) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human Calreticulin (CRT).
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Glu18-Ser193 with an N-Terminal His-Tag from human CRT, expressed in E. coli.
Shelf Life:1year
EU Commodity Code:30021010
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