84-1420-69 CGRP (CALCA, CALC1, Calcitonin Gene-related Peptide 1, Alpha-type CGRP, Calcitonin Gene-related Peptide I) 200ul 139518
特徴
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100-1:200Western Blot: 1:50-1:400Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-100 Immunocytochemistry: 1:50-1:500Optimal dilutions to be determined by the researcher.
Amino Acid Sequence:MGHHHHHHSGSEF- MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAFGR RRRDLQA
Positive Control (Supplied):139518A: Suppled as a recombinant protein (aa1-127) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.4, 0.01% sarcosyl, 1mM DTT, 5% trehalose, 0.05% Proclin 300
Specificity:Recognizes human Calcitonin Gene Related Peptide (CGRP)
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to aa1-127 with a N-terminal His-Tag from human CGRP, expressed in E. coli
Shelf Life:1year
EU Commodity Code:30021010
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