ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 modules) is a family of zinc-dependent proteases that are implicated in a variety of normal and pathological conditions, including arthritis and cancer.
ADAMTS protein fami.ly members contain an N-terminal propeptide domain, a metalloproteinase domain, a disintegrin-like domain and a C-terminus that contains a varying number of thrombospondin type-1 (TSP-1) motifs.
ADAMTS genes are primarily expressed in fetal tissues, including lung, kidney and liver.
ADAMTS-7 (ADAM metallopeptidase with thrombospondin type 1 motif, 7), also known as COMPase, is a 1686aa protein that exists as two alternatively spliced isoforms.
Encoded by a gene that maps to human chromosome 15q25.1, ADAMTS-7 contains eight TSP-1 motifs and binds one zinc ion per subunit.
ADAMTS-7 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
ADAMTS-7 is also located in meniscus, bone, tendon, cartilage, synovium, fat and ligaments, and is up-regulated in articular cartilage and synovium in arthritis patients.
ADAMTS-7 functions as a metalloprotease and may play a role in the degradation of COMP.
ADAMTS-7 is pH dependent, with optimum pH between 7.5 and 9.5.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunocytochemistry: 5-20ug/mlImmunohistochemistry (Frozen): 5-20ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: 139017A: A Disintegrin And Metalloproteinase With Thrombospondin 7, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding Val1589~Arg1686 of human A Disintegrin And Metalloproteinase With Thrombospondin 7, expressed in E. coli.
