Protein A is a 40-60kD MSCRAMM (microbial surface components recognizing adhesive matrix molecules) surface protein originally found in the cell wall of the bacteria Staphylococcus aureus.
It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR.
It has found use in biochemical research because of its ability to bind immunoglobulins.
It binds proteins from many of mammalian species, most notably IgGs.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：chicken
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity：Recognizes one precipitin arc against Staphylococcal Protein A.
