B1--Adrenergic Receptor (B1AR) is a G protein-coupled receptor (GPCR) involved in the regulation of cardiovascular functions (1).
Together with B2AR, B1AR is a major BAR in the heart.
B1AR is activated by catecholamines and couples to Gas protein, activating adenylate cyclase and increasing intracellular cAMP levels (2).
Beta-blockers (BAR antagonists), one of the major class of therapeutics in cardiovascular medicine, act mostly by preventing catecholamine binding to B1AR (3).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human B1-Adrenergic Receptor protein. Species Crossreactivity: mouse, rat. Does not cross-react with B2-Adrenergic Receptor.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Gly406 of human B1-Adrenergic Receptor. Species Sequence Homology: monkey
