84-1411-06　Ferritin H-chain (FTH) (BSA, Azide, Glycerol Free) 50ug　F4015-40A

特徴

• Elemental iron is required for a variety of normal cellular functions and is vital for proper growth and development.
• Ferritin is the major protein involved in iron sequestration and detoxification.
• Ferritin is found in all living species.
• Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL).
• The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively.
• Ferritin molecules from plants and bacteria contain only H-type chains, where 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms.
• FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3 and FTH subunit (rich in human heart) is located on chromosome 11.
• Ferritin is capable of storing up to 4,500 atoms of ferric iron.
• The H-to-L ratio within ferritin varies in a tissue-specific manner and is also influenced by pathophysiological conditions, including inflammation and malignancy.
• Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs.
• H-chains are important for Fe(II) oxidation and L-chains assist in core formation.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1ug/ml.
• Western Blot: 1:1000-1:5000 using ECLOptimal dilutions to be determined by the researcher.
• Positive Control for Western Blot:F4015-55: Ferritin H-chain, Human, Western Blot Pos.
• Control
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.