84-1410-82　［取扱停止］OGG1 (N-glycosylase/DNA Lyase, 8-oxoguanine DNA Glycosylase, DNA-(Apurinic or Apyrimidinic Site) Lyase, AP Lyase, MMH, MUTM, OGH1) 100ul　136942

特徴

• 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion.
• In E.
• coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine.
• One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue.
• hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes.
• Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development.
• The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers.
• In particular, 3p25-p26 is a common LOH region in lung cancer.
• Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases.
• Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage.
• Thus, Ogg provides a direct link between DNA damage and toxicity in neurons.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:1000Immunohistochemistry (Frozen): 1:25-1:100Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.