Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic and have reduced activity, metabolism, and body temperature.
Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively.
The expression of leptin mRNA is restricted to adipose tissue.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 2-15ug/mlWestern Blot: 0.1-0.2ug/mlELISA: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS.
Specificity：Recognizes mouse Leptin. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to mouse leptin 16kD expressed in E. coli.
