alpha-internexin is a ~66kD Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits.
It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization.
It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes.
Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H.
In some mature neurons alpha-internexin is the only neurofilament subunit expressed.
Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture.
In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1-1:000Western Blot: 1:10,000-1:20,000 (brain or spinal cord homogenate; look for a major band at SDS-PAGE apparent MW of 66-68kD, depending on the species.
The human protein runs at ~68kD, while rat, mouse and other species run at ~66kD.
Minor bands at ~150kD are probably covalent dimers, and bands at ~50kD represent a-internexin breakdown products.)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes NF66. Species Crossreactivity: human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant corresponding to alpha-internexin.
