Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor.
VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases.
Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:100Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Positive Controls:Rat astrocytes and C6 and U251 gliomas
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-citrate/phosphate, pH 7-8, 0.09% sodium azide.
Specificity：Recognizes rat Factor Inhibiting HIF-1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to rat Factor Inhibiting HIF-1, from the N-terminal extracellular domain conjugated to immunogenic carrier.
