GAP-43 (growth associated protein 43kD; also known as neuromodulin) is a 24kD, neuron growth cone-associated member of the neuromodulin family.
Apparently due to its tertiary structure, it runs anomalously at 43kD on SDS-polyacrylamide gel.
Rat GAP-43 is a 226aa protein that contains an N-terminal lipid binding motif, an 11aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa38-48), and a C-terminus with four potential serine phosphorylation sites.
The molecule may utilize the IQ domain to bind calmodulin.
Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction.
Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Recognizes rat GAP43. Species Crossreactivity: mouse and human.
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa216-226 from rat GAP-43, (C)-KEDPEADQEHA coupled to maleimide-activated KLH.
