Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells.
There are seven types of glucose transport carrier proteins, designated as Glut 1-7.
Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of aa homology (45%-65%), all in the MW range of 40-60kD.
Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli.
In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes, as in blood brain barrier and blood testis barriers.
GLUT1 is a major glucose transporter in the mammalian blood brain barrier.
It is ubiquitous, and is present at high levels in primate erythrocytes and brain endothelial cells.
Applications:Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:100-1:250Western Blot: 0.5-2ug/mlImmunocytochemistry: 1:50-1:200Immunofluorescence: 1:500-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human GLUT1. Species Crossreactivity: bovine, mouse, primate, rat and rabbit.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Bo Hu Mo Rb Rt
Immunogen：Synthetic peptide corresponding to aa300-400 from the internal region of human LPIN1.
