Corticotropin Releasing Hormone Receptor (CRF) is a member of the corticotropin-releasing factor family of G-protein-coupled receptors that also includes CRF It is abundantly expressed in the CNS with major expression in the cortex, cerebellum, hippocampus, amygdala, olfactory bulb and pituitary.
In the periphery, the receptors are expressed at low levels in the skin, ovary, testis and adrenal gland.
It mediates stress and anxiety including stress related colonic motor activity.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:4000-1:8000 (PBS/0.3% Triton X-100 using Bn/Av-HRP technique)Immunofluorescence (indirect): 1:200-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 100ul distilled or deionized dH2O.
Specificity：Recognizes rat Corticotropin Releasing Hormone Receptor.
