84-1410-53　CGRP (CALCA, CALC1, Calcitonin Gene-related Peptide 1, Alpha-type CGRP, Calcitonin Gene-related Peptide I) 100ul　136882

特徴

• Calcitonin gene-related peptide (CGRP) is present in C-cells of the thyroid and in central and peripheral nerves.
• CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
• Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100-1:200 (Fluorescein), 1:1000-1:2000 (Streptavidin/HRP)Immunohistochemistry: 10um cryostats.
• Fixative: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4; 500 mL over ~20-30min.
• Post Fixation: 1.5h at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.