The CKI family consists of several different genes, divided in alpha, beta, gamma, delta and epsilon, some of which are encoded by more than one gene.
The single mammalian CK1 alpha gene can generate four different proteins by alterate transcription.
CK1a was raised against the shortest of these proteins, and will therefore bind to all CK1 isotypes.
The CK1 alpha proteins are involved in the control of protein degradation, and phosphorylate acidic regions of their substrate molecules.
Typically the target ser/thr residues closely C-terminal to two or three aspartic or glutamic acid residues.
Alternately, phosphorylation on residues 3 or 4aa N-terminal to a ser/thr residue can also favor CKI phosphorylation.
This means that CKI family enzymes often phosphorylate one or more ser/thr residues C-terminal to a phosphorylation site generated by the activity of another protein kinase.
CK1 alpha is plays a role in Alzheimer's and Sporadic inclusion body myositis.
Expression of the gene leads to accumulation of tau phospho-epitopes in both diseases.
It is also suspected to be an apoptosis suppressor by binding to MDM2 and down regulating p53.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:1000Western Blot: 1:10,000Immunocytochemistry: 1:5000 (ABC or other enzyme linked immunocytochemical procedures)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS.
Specificity：Recognizes human Casein Kinase 1 alpha.
