Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage.
Aggrecan is composed of three major domains: G1, G2, and G3.
Between the G1 and G2 domains there is an interglobulin region (IGD).
The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase (ADAMTS).
Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis.
The C-terminal NITEGE fragment is one of the G1 fragments produced when aggrecan is cleaved with aggrecanase.
It is the most abundant fragment found in normal human synovial fluid and articular cartilage, and is the major product produced by aggrecanase cleavage.
The NITEGE fragment has also been shown to accumulate rapidly after an acute cartilage injury.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1ug/mlWestern Blot: 1ug/ml (band is detected at ~61kD representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 30% glycerol.
Specificity：Recognizes human Aggrecan. Species Crossreactivity: mouse, rat, bovine, feline and porcine
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Bo Fe Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C G G N (aa387) and I T E G E (aa392) of human aggrecan.
