Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins.
Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP.
The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons.
Adenylate cyclase type III is a large complex molecule of, in the human, 1145aa with a deduced molecular weight of 129kD.
The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kD in cortex and about 200kD in olfactory epithelium.
The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains.
Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:1000Western Blot: 1:10,000 (major bands at 160-200kD, depending on the tissue)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes rat ADCY3. Species Crossreactivity: feline, chicken, human and mouse.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Ch Fe Hu Mo Rt
Immunogen：Recombinant corresponding to fusion protein containing the extreme C-terminal segment of rat NF-M.
