84-1410-45　RAB4A (Ras-related Protein Rab-4A, Oncogene Rab4, RAB4) 25ul　136840

特徴

• Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily.
• Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell.
• Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states.
• This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2).
• Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3).
• Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1).
• Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-terminal domains with a cysteine motif implicated in sub-cellular targeting.
• Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3).
• Each Rab shows a characteristic sub-cellular distribution (4).
• In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments.
• The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes.
• Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5).
• Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus.
• Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.