The 21kD guanine-nucleotide binding proteins (KRas, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms).
Receptor tyrosine kinases and G-protein -coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway.
GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras.
However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway.
The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol
Specificity：Recognizes human RAS. Species Crossreactivity: mouse, rat and bovine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa31-43 from human RAS. Sequence Homology: yeast, slime mould, fungi, Xenopus, rat, mouse and chicken
