Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kD (Hsp10s).
Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides.
Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process.
Cpn10 has been located in human platelets, but is also present in human maternal serum.
It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development.
This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide.
Specificity：Recognizes human Cpn10 at ~10kD. Species Crossreactivity: mouse, rat, bovine, canine, guinea pig, porcine, rabbit, sheep and Xenopus.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC IP WB
Calc Crossreactivity：Bo Ca Gp Hu Mo Po Rb Rt Sh Xe
Immunogen：Synthetic peptide corresponding to aa91-101 from human Cpn10.Cellular Localization: Mitochondrion Matrix.
