Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues.
It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3).
In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta.
They are structurally similar, but functionally non-identical.
GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases.
GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4).
GSK-3 alpha and GSK-3 beta share 85% amino acid identity.
GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer’s, cancer and bipolar (4, 5).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4.
Specificity：Recognizes human GSK-3. Species Crossreactivity: mouse, rat, boine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human GSK-3Beta at C-terminal.
