Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes.
Known as water channels, they are integral membrane pore proteins (1, 2).
Aquaporin 4 is constitutively expressed in the basolateral cell membrane of princinpal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3).
It is also expressed in astrocytes and is upregulated by direct insult to the CNS (4).
Aquaporin 4 has been propsed as the primary autoimmune target in neuromyelitis optica ( 5, 6).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:400Western Blot: 1:1000-1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Affinity Purified
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat Aquaporin 4 at ~35kD. May detect larger glycosylated band at ~50kD. Species Crossreactivity: Human and mouse.
