Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils.
Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation.
Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2).
These include the amyloid-b peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer’s symptomology, the deposition of a-synuclein in the Lewy bodies of Parkinson’s disease, and accumulation of polyglutamine-containing aggregates in Huntington’s disease (2, 3).
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry, ELISA, Western Blot, Dot Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1;100-1:1000Dot Blot: 1;100-1:1000Immunohistochemistry (Formalin fixed): 1;1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes all types of amyloid oligomers. Recognizes a peptide backbone epitope that is common to amyloid oligomers, but not found in native proteins, amyloidgenic monomer or mature amyloid fibrils. Species Crossreactivity: Eukaryotes, human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：DB E IC IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic molecular mimic of soluble oligomers.
