84-1410-13　［取扱停止］VDR (NR1I1, Vitamin D3 receptor, 1,25-dihydroxyvitamin D3 receptor) 50ug　136518

特徴

• The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D.
• The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone.
• The vitamin D receptor affects growth, bone formation, and female reproduction.
• For example, this receptor modulates the growth and differentiation of keratinocytes and mediates the beneficial effects of vitamin D on psoriatic skin.
• It also inhibits hormone secretion by and proliferation of parathyroid cells.
• The vitamin D receptor functions either as a homodimer or as a heterodimer of vitamin D and retinoid acid X receptor subunits.
• Inactivation of the receptor leads to hypocalcemia and Rickets-alopecia syndrome.
• Recently, the vitamin D receptor has been shown to function as a receptor for the secondary bile acid lithocholic acid (LCA), which is hepatotoxic and a potential enteric carcinogen.
• Activation of VDR by LCA or vitamin D induced expression in vivo of CYP3A, a cytochrome P450 enzyme that detoxifies LCA in the liver and intestine.
• An alternatively spliced vitamin D receptor that has an altered ligand-binding domain has been isolated from rats.
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100-1:500 ELISA: 1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.