Tissue transglutaminase is an enzyme which catalyzes the cross linking of proteins and the conjugation of polyamines to proteins.
While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent.
Transglutaminase 5 contributes to the formation of the cornified cell envelope of keratinocytes.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml Immunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:Recognizes human TGM5/Transglutaminase 5. Species Crossreactivity: Mouse, rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to human TGM5/Transglutaminase 5 at C-Terminal.