Synaptophysin (Syn1) is an abundant synaptic vesicle membrane protein that contains four transmembrane regions.
It forms a high molecular weight complex in the vesicle membrane containing at least four Syn I subunits and an 18kD protein termed synaptobrevin/VAMP.
Syn I is phosphorylated by both serine/threonine and tyrosine kinases on its cytoplasmic tail.
Knockout studies in mice have shown that it is not essential for neurotransmitter release.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SYP/Synaptophysin. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human SYP/Synaptophysin.
