Necessary for the ATP-dependent first step of spliceosome assembly.
Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA.
May act as transcription repressor.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500ELISA: 1:20,000Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:100 & 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human SF1. Species Crossreactivity: Mouse
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide corresponding to human SF1 at N-Terminal.