PTP1B, a NT1 non-receptor protein tyrosine phosphatase, has been shown to regulate insulin signaling, integrin signaling, and leptin signaling.
It has been shown that JAK2 and TYK2 are physiological substrates of PTP1B and that the substrate recognition site within theses kinases is similar to the site of dephosphorylation identified within the insulin receptor.
The knowledge that mice lacking PTP1B are hypersensitive to insulin and resistant to obesity and that insulin signaling can be enhanced by the inhibition of PTP1B have made PTP1B a potential therapy target against type 2 diabetes and obesity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELSA: 1:20,000Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTPN1/PTP1B. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PTPN1/PTP1B at N-Terminal.
