The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion.
At least eleven isozymes have been described.
PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V).
The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain.
The conventional PKC subfamily (a, aI, aII, and a) is regulated by both Ca2+ and diacylglycerol.
The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors.
The phosphorylation of multiple sites in conventional PKCs regulates their activity.
In mast cells, FcaRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCaI and PKCa, respectively.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Immunohistochemistry (Paraffin): 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Specificity：Recognizes human PRKCG/PKC-Gamma. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PRKCG/PKC-Gamma.
