This gene encodes a protein localized to the nucleus in proliferating cells.
Expression of this gene is modulated by cytokines in natural killer and T cells.
The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
Two transcripts encoding different isoforms have been identified.
PDCD4 inhibits the translation initiation factor E1F4A.
Phosphorylation by AKT causes nuclear translocation of PDCD4 and also decreases the ability of PDCD4 to interfere with the transactivation of AP-1 responsive promoter by c-JUN.
Loss of PDCD4 expression in human lung carcinoma correlates with tumor progression and prognosis.
Applications:Suitable for use in Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200Immunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PDCD4. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa458-468 from human PDCD4 (GDGGRLKPESY).
