PAK1, a PAK (STE20) type protein kinase, is an effector for the small GTP binding proteins Cdc42 and Rac in the regulation of cell motility and morphology.
The activated protein has been shown to act on a variety of targets, including actin-binding protein filamin A, myosin light chain kinase, histone H3 and the stress-activated protein kinase JNK1.
The PAK1 gene has been mapped to chromosome 11q13, a region of amplification in a subset of estrogen receptor positive breast carcinomas prone to metastasis.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500 ELISA: 1:1000Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PAK1. Species Crossreactivity: Mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PAK1.
