The members of this family encode proteins with 50 to 80% sequence identity to each other.
The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls.
The MAGEA genes are clustered at chromosomal location Xq28.
They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
MAGE was originally isolated from human melanoma cells as an antigen that is recognizes by cytolytic T lymphocytes.
It has since been documented in other carcinomas.
In addition, expression has been documented in highly proliferating germ cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000 Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MAGEA1/MAGE 1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human MAGEA1/MAGE 1 at C-Terminal.
