Kir6.2/Kcnj11, an ATP sensitive inwardly-rectifying potassium channel, was originally isolated from an insulinoma library.
Actual conductance requires association with the sulfonylurea receptor.
This receptor is inhibited by sulfonylureas and activated by diazoxide.
Mutations in the KNCJ11 gene are found in patients with permanent diabetes mellitus of infancy and hyperinsulinemic hypoglycemia.
It is thought that this channel couples metabolism to membrane electrical events fibringing about insulin secretion.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500ELISA: 1:10,000Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KCNJ11/Kir6.2. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human KCNJ11/Kir6.2.
