Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter.
Heme oxygenase activity is induced by its substrate heme and by various nonheme substances.
Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2.
HMOX1 and HMOX2 belong to the heme oxygenase family.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human HMOX-1. Species Crossreactivity: Canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Ca Gp Hm Hu Mk Mo Po Rb Rt Sh
Immunogen：Recombinant corresponding to human HMOX-1 (Hsp32).
