Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase; PGH synthase; Cox-1) is a key enzyme in prostaglandin biosynthesis.
The first rate-limiting step in the conversion of arachidonic acid to prostaglandins is catalyzed by Cox-1 and Cox-2.
The cyclooxygenase activity of Cox-1 is inhibited by nonsteroidal antiinflammatory drugs such as aspirin and endomethacin, though it is not a mitogen-inducible gene.
It is a housekeeping gene encoded by a 2.8-kb mRNA, and is an integral membrane protein located primarily in the endoplasmic reticulum.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:40,000Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PTGS1/COX-1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human PTGS1/COX-1 at C-Terminal.
