Caspase 9 is a cysteine C14 endopeptidase that mediates oncogene and drug-induced apoptosis.
It is activated upon binding to Apaf-1/cytochrome c and inhibited upon phosphorylation by the serine/ threonine kinase Akt.
Activated caspase-9 cleaves downstream caspases such as Caspase-3, -6 and -7 initiating the caspase cascade.
Like other caspases, Caspase-9 plays a critical role in mediating apoptosis during normal brain development.
Caspase-9 also cleaves amyloid beta-protein precursors, principal constituent of senile plaques, and is involved in neuronal death observed in Alzheimer disease.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000 Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Caspase 9.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Caspase 9.
