Applications:Suitable for Western Blot, Dot Blot, ELISA, Immunoprecipitation and Conjugation.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:16,000 1ug of Glutamine Synthetase was used in a standard sandwich ELISA with Rabbit anti-Goat (HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes microbial Gluramine Synthase. Assay by IEP resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase.
