84-1332-41　AES (Amino-terminal Enhancer of Split, Amino Enhancer of Split, Gp130-Associated Protein GAM, Protein ESP1, Protein GRG, GRG) 100ul　123037

特徴

• Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading.
• Anoikis is the molecular mechanism of apoptosis induced by integrin detachment.
• Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co- repressors that play important roles in neurogenesis, segmentation, and sex determination.
• AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis.
• It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family.
• Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the a5b1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the a5b1 integrin.
• Applications:Suitable for use in Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MCHKNGFPQEGGITAAFLQKRKLRLSKNHRPARAKVTEHVRGTRPGRATAGPAASTRAAGSLFFDRWGNRGPAGCRGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQAHLSKEDKNGHDGDTHQEDDGEKSD
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.