Applications:Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5,000-1:100,000 using chromogenic substratesImmunocytochemistry: 1:500-1:5,000Immunohistochemistry: 1:500-1:5,000Western Blot: 1:5,000-1:100,000 using chromogenic substratesWestern Blot: 1:10,000-1:200,000 using ECL substratesOptimal dilution to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：donkey
Source Antibody：goat
Grade：Affinity Purified
Purity：Purifed by immunoaffintiy chromatography
Form：Supplied as a powder lyophilized from a solution containing PBS, pH 7.6, and 15mg/ml BSA (IgG-free and protease-free). Labeled with Horseradish Peroxidase (HRP). Reconstitute with 0.5ml ddH2O.
Specificity：Reacts with whole molecule goat IgG. Also reacts with the light chains of other goat immunoglobulins. Has been solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, equine, human, mouse, rabbit, and rat serum proteins, but it may cross-react with immunoglobulins from other species.
