Interleukin 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts, and epithelial cells.
It has a wide variety of biological functions including activating cell surface signaling assemblies composed of IL6, IL6RA, and the shared signaling receptor gp130.
The cytokine plays an essential role in the final differentiation of b-cells into Ig secreting cells and induces myeloma, plasmacytoma growth, and nerve cell differentiation.
In addition, IL-6 is an important regulator of acute phase reactions.
Ota et al.
(2001) presented evidence that variations in IL-6 may be related to osteoporosis.
Applications:Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Detection): 0.1-0.4ug/mlImmunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensinWestern Blot: 0.1ug/ml using recombinant human IL-6 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a powder PBS, pH 7.4, 50ug BSA, 1ug carrier protein. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes human IL-6. In ELISA, <0.1% crossreactivity with recombinant porcine IL6 and <0.01% crossreactivity with recombinant mouse IL6 and recombinant rat IL6 is observed.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro29-Met212 from human IL6, expressed in E. coli. Uniprot/Swiss Accession: P05231
