Penicillinase is a specific type of β-lactamase, showing specificity for penicillins, again by hydrolysing the beta-lactam ring.
Molecular weights of the various penicillinases tend to cluster near 50kD.
Penicillinase was the first β-lactamase to be identified: It was first isolated by Abraham and Chain in 1940 from Gram-negative E.
coli even before penicillin entered clinical use,[2] but penicillinase production quickly spread to bacteria that previously did not produce it or produced it only rarely.
Penicillinase-resistant beta-lactams such as methicillin were developed, but there is now widespread resistance to even these.
Penicillinases are classified as Group 2 along with cephalosporinases, both are inhibited by clavulanic acid, corresponding to the molecular classes A and D reflecting the original TEM and SHV genes.
Due to the increasing number of TEM- and SHV-derived β-lactamases, Penicillinases are further divided into the subclass 2a.
Applications:Suitable for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence as well as other antibody based assays using streptavidin or avidin conjugates.
Recommended Dilution:ELISA: 1:2000 to 1:10,000.
1ug of Penicillinase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, before the addition of glycerol to 40%. Labeled with biotin.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
