Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorecscence, Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000- 1:50,000
Western Blot: 1:2,000- 1:10,000
Immunohistochemistry : 1:1,000- 1:5,000.
Frozen tissue sections, paraffin-embedded tissues, and 4% paraformaldehyde-fixed tissues
Immunofluorescence: 1:5,000 -1: 10,000
Optimal dilutions to be determined by the researcher.
Label:Biotin
Biotin/Protein Ratio:10-20 Biotin molecules per rabbit IgG molecule.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide Labeled with Biotin.
Specificity：Recognizes a 464kD band representing E. coli beta-Galactosidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti biotin, anti rabbit serum and purified and partially purified β-Galactosidase (E. coli).
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Ec
Immunogen：Affinity purified beta-galactosidase (E. coli).
