Applications:Suitable for Western Blot and ELISA..
Other applications not tested.
Recommended Dilution:ELISA: 1:8000-1:32,000.
Assayed against 1ug Acid Phosphatase in standard capture ELISA using Streptavidin (HRP) (Catalog No: S7973-97A) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (Catalog No: A0030)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Also available:A0560: Acid Phosphatase (Potato) Pab Rb x (antiserum)A0560-05: Acid Phosphatase (Potato) Pab Rb x (Highly purified)A0560-06: Acid Phosphatase (Potato) (Biotin) Pab Rb xA0560-07: Acid Phosphatase (Potato) (HRP) Pab Rb x
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glcyerol. No stabilizing proteins added. Labeled with Biotinyl N-hydroxysuccinimide ester (BNOHSA, Biotin). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Specificity：Recognizes Acid Phosphatase. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Acid Phosphatase (Potato).
