Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPAR?, a master regulator of adipocyte differentiation, and to control adipogenesis.
It disrupts insulin signaling by binding directly to Akt kinases and blocking their activation.
It interacts also with the NF-kappaB transactivator p65 RELA.
May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells.
Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Western Blot: 1:2000-1:5000 using ECL.
Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureImmunohistochemistry (paraffin sections): 1:500Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human TRB-3. Detects a band of ~28kD by Western Blot (predicted ~39kD).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa112-125 (ARLPPHKHVARPTE) of human TRB-3.
