RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-kappaB involved in the regulation of key genes in rapid cellular activation responses.
RpS3 interacts with nm23-H1.
The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells.
The phosphorylated ERK is reduced by the expression of rpS3.
Applications:Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct and indirect): 1:2000-1:5000Immunocytochemistry: 1:100Immunohistochemistry: 5ug/mlImmunoprecipitation: 1:500-1:5000Western Blot: 1:500-1:5000 using ECL.
Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk.
Suggested incubation time is 1 hour at room temperatureOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Specificity：Recognizes human ribosomal protein S3. Detects a band of ~32kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ribosomal protein S3.
